human tumour cell lines jurkat  (DSMZ)

Journal: Molecules

Article Title: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

doi: 10.3390/molecules25040964

Figure Lengend Snippet: Cytotoxicity of bersavine and berbamine following single-dose exposure at a concentration of 10 μM. Doxorubicin at 1 µM was used as a reference drug. Data are shown as mean values ± SD of at least three independent experiments and are expressed as the percent of proliferation of 0.1% dimethyl sulfoxide (DMSO) mock-treated control cells (100%).

Article Snippet: The selected human tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukaemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and HepG2 (hepatocellular carcinoma) were purchased from either ATCC (Manassas, VA, USA) or Sigma-Aldrich (St. Louis, MO, USA) and were cultured in accordance with the provider ́s culture method guidelines.

Techniques: Concentration Assay, Control

Journal: Molecules

Article Title: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

doi: 10.3390/molecules25040964

Figure Lengend Snippet: The effect of bersavine on the proliferation and viability of Jurkat and MOLT-4 leukemic cells. Changes in proliferation ( A ) and viability ( B ) were monitored for 24 and 48 h of treatment by Trypan blue exclusion analysis. Results are shown as the mean ± SD from . Cells treated with 5 µM of cisplatin were used as a positive control. * Significantly different to control ( p ≤ 0.05).

Article Snippet: The selected human tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukaemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and HepG2 (hepatocellular carcinoma) were purchased from either ATCC (Manassas, VA, USA) or Sigma-Aldrich (St. Louis, MO, USA) and were cultured in accordance with the provider ́s culture method guidelines.

Techniques: Positive Control, Control

Journal: Molecules

Article Title: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

doi: 10.3390/molecules25040964

Figure Lengend Snippet: Analysis of the cell cycle after treatment with bersavine. The figure shows representative histograms of Jurkat ( A ) and MOLT-4 ( B ) leukemic cells at a 24 h interval with a mean percentage of cells cycling through phases G1, S, and G2 from a flow cytometry measurement of three separate treatments. The bar graph summarizes cumulative data on the percentage of cells in each phase of the cell cycle. Data are presented as mean values ± SD, n = 3. * Significantly different to control ( p ≤ 0.05).

Article Snippet: The selected human tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukaemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and HepG2 (hepatocellular carcinoma) were purchased from either ATCC (Manassas, VA, USA) or Sigma-Aldrich (St. Louis, MO, USA) and were cultured in accordance with the provider ́s culture method guidelines.

Techniques: Flow Cytometry, Control

Journal: Molecules

Article Title: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

doi: 10.3390/molecules25040964

Figure Lengend Snippet: The effect of bersavine on the induction of apoptosis. Apoptosis was determined by Annexin V and propidium iodide (PI) staining 24 h after treatment of Jurkat ( A ) and MOLT-4 ( B ) leukemic cells. Representative histograms of one of three independent measurements are shown. Cells treated with 5 µM of cisplatin were used as a positive control. The bar graph represents the percentage of early and late apoptotic cells detected by flow cytometry (mean ± SD, n = 3). * Significantly different to control for early (Annexin V+) apoptotic cells ( p ≤ 0.05). # Significantly different to control for late (Annexin V/PI+) apoptotic cells ( p ≤ 0.05).

Article Snippet: The selected human tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukaemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and HepG2 (hepatocellular carcinoma) were purchased from either ATCC (Manassas, VA, USA) or Sigma-Aldrich (St. Louis, MO, USA) and were cultured in accordance with the provider ́s culture method guidelines.

Techniques: Staining, Positive Control, Flow Cytometry, Control

Journal: Molecules

Article Title: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

doi: 10.3390/molecules25040964

Figure Lengend Snippet: The effect of bersavine on the activity of caspases using luminescence-based assays. The activity of caspases-3/7, caspase-8 and caspase-9 were determined in Jurkat ( A ) and MOLT-4 ( B ) cells 24 and 48 h after treatment. Caspase activities are represented as luminescence intensity. Data are presented as mean values ± SD, n = 3. * Significantly different to control ( p ≤ 0.05).

Article Snippet: The selected human tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukaemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and HepG2 (hepatocellular carcinoma) were purchased from either ATCC (Manassas, VA, USA) or Sigma-Aldrich (St. Louis, MO, USA) and were cultured in accordance with the provider ́s culture method guidelines.

Techniques: Activity Assay, Control

Journal: Molecules

Article Title: Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells

doi: 10.3390/molecules25040964

Figure Lengend Snippet: Western blot analysis of proteins that regulate checkpoint responses or induction of cell death in MOLT-4 and Jurkat leukemic cells upon treatment with bersavine. MOLT-4 and Jurkat cells were treated with bersavine for 24 h. Control cells were mock treated with 0.1% DMSO (DMSO) and 5 μM of cisplatin-treated cells were used as a positive control (PC). These experiments were performed at least three times with similar results and a cropped blot is shown from one representative experiment.

Article Snippet: The selected human tumour cell lines Jurkat (acute T cell leukemia), MOLT-4 (acute lymphoblastic leukaemia), A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), PANC-1 (pancreas epithelioid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), SAOS-2 (osteosarcoma) and HepG2 (hepatocellular carcinoma) were purchased from either ATCC (Manassas, VA, USA) or Sigma-Aldrich (St. Louis, MO, USA) and were cultured in accordance with the provider ́s culture method guidelines.

Techniques: Western Blot, Control, Positive Control